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The Application of QuEChERS in the Extraction of Anabolic Steroids in Whole Blood

Application Notes UCT-SP08:

Anabolic steroids are drugs structurally related to the cyclic steroid ring system and behave similarly to testosterone in the body. Anabolic steroids are used therapeutically to stimulate muscle growth and appetite, induce male puberty and treat chronic wasting conditions, such as cancer and AIDS [1]. Ergogenic uses of anabolic steroids include bodybuilding, sport doping, and animal fattening. Developing a fast, simple and effective analytical method for anabolic steroids in complex biological samples is of great interest in clinical, anti-doping and food safety testing labs. This application utilizes the original non-buffered QuEChERS (acronym for Quick, Easy, Cheap, Effective, Rugged and Safe) technique to quatify anabolic steroids in human whole blood. Previous extraction techniques typically involved a protein precipitation step followed by liquid-liquid extraction (LLE) or solid phase extraction (SPE). 

This application note was authored by UCT. Please complete the information in the "Download Application Note" box to download the complete article.